Cell cycle kinetics of irradiated synchronous and asynchronous tumor cells with DNA distribution analysis and BrdUrd-Hoechst 33258-technique
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چکیده
منابع مشابه
Mouse-human heterokaryon analysis with a 33258 Hoechst-Giemsa technique
The bibenzimidazol derivative 33258 Hoechst can be used to distinguish microfluorometrically between mouse and human nuclei in heterokaryons. This affords a quick and accurate alternative to autoradiography in the analysis of such heterokaryons. The 33258 Hoechst fluorescence patterns can be converted after irradiation to a Giemsa rendition of the differential staining.
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Hoechst 33342 stain has been used previously in conjunction with fluorescence activated cell sorting to separate cell populations into the various phases of the cell cycle. The present experiments were designed to evaluate the toxicity of this stain in cells derived from solid KHT sarcomas which were untreated or irradiated prior to dissociation. In both untreated and irradiated cells, stain to...
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ژورنال
عنوان ژورنال: Cytometry
سال: 1981
ISSN: 0196-4763,1097-0320
DOI: 10.1002/cyto.990020206